The information compiled in this workbook is taken from both international and Indian experts engaged in assisted reproductive procedures. The 17 chapters included in this book contain practical information, step by step protocols, list of supplies and equipment and selected references. The field of human-assisted reproduction is growing by leaps and bounds with recent advances in areas of embryo culture, vitrification, Preimplantation genetic diagnosis (PGD), new WHO guidelines for semen examination and the latest techniques for the surgical retrieval and methods for the selection of best spermatozoa. However, the advances in this promising field are being severely threatened due to the lack of rigorous training and availability of laboratory personnel qualified to work in the human embryology clinics in less developed or in more developed countries. This manual will be of immediate help to all those interested in the field of assisted reproductive technologies (ARTs). The readers can learn and master various sophisticated techniques included in this manual and in turn contribute to improvements in ART outcome of individual clinics.