Background & Objectives: Ascitic fluid infections are frequent complications among patients of cirrhosis with ascities, of which spontaneous bacterial peritonitis (SBP) is the most common and potentially fatal. This study was planned to know the etiology and current antibiotic susceptibility profile of the isolates from the SBP patients. Material and Methods: A total of 50 cases of SBP from cirrhosis patients with ascites were enrolled in this study. Samples were processed in automated Bactec or Bac-T /Alert. Further identification & antimicrobial susceptibility testing was done by VITEK-2 system. Results: Most common organism isolated was Escherichia coli (40%) followed by Coagulase negative Staphylococci, Klebsiella pneumonia and Acinetobacter baumanii. Among all Gram negative isolates, 94.7% were sensitive to tigecycline, 92.1% sensitive to colistin. It also showed high susceptibility to amikacin and carbapenems while low susceptibility was seen to others. All the gram positive organisms were sensitive to vancomycin & linezolid and show moderate sensitivity to ciprofloxacin, tetracycline, cotrimoxazole, and gentamycin. Low sensitivity was seen to penicillin. Methicillin resistant coagulase negative Staphylococci (MRCoNS) were seen in 4 (57%) isolates. Interpretation & Conclusion: Escherichia coli is the most common cause of SBP. Antimicrobial resistance is increasing therefore early detection and determination of antimicrobial susceptibility pattern is important to reduce the mortality and morbidity associated with ascitic fluid infections.