Acute diarrhea is a common problem in children in developing countries like India leading to hospitalization. The objective of the studywas to assess the etiological agents causing diarrhea in children and their sensitivity patterns to various antimicrobials. This study was carried out from January 2007- December 2009 on 860 pediatric patients attending the out patients department or admitted in thewards of Lok Nayak Hospital, New Delhi with gastroenteritis. Eight hundred and sixty stool samples were collected and examined for various pathogens. Enteric pathogens were isolated from 321 (37.2%) samples. Parasitic pathogens were observed in 67 (7.79%) stool samples. V.cholerae was the most commonly isolated bacteria 134/321 (41.74%), followed by Shigella 90/321 (28.03%) and E.coli 86 (27.79%). Salmonella spp. (1.5%) and Aeromonas hydrophila (1.86%) were isolated less frequently. Resistance to amoxycillin was observed in 99% of Vibrio, 95% of E. coli, 58% of Shigella and 20% of Salmonella species. Variable degree of resistance ranging from 0% in Salmonella to 69% in E. coli was observed against ciprofloxacin, while resistance to nalidixic acid was observed in more than 90% of all types of bacterial pathogens. Resistance to ceftriaxone, cefotaxime and gentamicin was less common. Identification of the etiological agent of diarrhea in children is very important as it can help in the institution of appropriate therapy and the reduction ofmorbidity andmortality in these patients.