Journal of Gastrointestinal Infections

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VOLUME 6 , ISSUE 1 ( 2016 ) > List of Articles

REVIEW ARTICLE

Pattern of antibiotic resistance in Helicobacter pylori

Atul Sachdev, Preeti Chaudhary, Varsha Gupta

Citation Information : Sachdev A, Chaudhary P, Gupta V. Pattern of antibiotic resistance in Helicobacter pylori. J Gastrointest Infect 2016; 6 (1):16-21.

DOI: 10.5005/jp-jogi-6-1-16

License: CC BY-SA 4.0

Published Online: 01-06-2015

Copyright Statement:  Copyright © 2016; The Author(s).


Abstract

Antibiotic resistance has been an emerging concern for common bacterial infections worldwide. Helicobacter pylori, commonly associated with chronic bacterial infections, is also included in bacteria with drug resistant problems. Its infection, once considered curable, is now becoming a matter of grave concern with rising antibiotic resistant patterns reported worldwide. Resistance is mainly reported to the key antibiotics in the treatment of infection i.e. metronidazole and clarithromycin, and to a lesser extent to amoxicillin and tetracyclin, thereby decreasing the cure rates of the combination therapies used. Recently resistance to quinolones has also been reported.


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  1. “All Nobel Prizes in Physiology or Medicine”. Nobelprize.org. Nobel Media AB 2014; Available from .
  2. Malfertheiner P, Megraud F, O'Morain CA, Atherton J, Axon AT, Bazzoli F et al. Management of Helicobacter pylori infection the Maastricht IV/ Florence Consensus Report. Gut. 2012;61:646-64.
  3. McMahon BJ, Hennessy TW, Bensler JM, Bruden DL, Parkinson AJ, Morris JM. The relationship among previous antimicrobial use, antimicrobial resistance, and treatment outcomes for Helicobacter pylori infections. Ann Intern Med. 2003;139:463-69.
  4. Datta S, Chattopadhyay S, Nair GB, Mukhopadhyay AK, Hembram J, Berg DE et al. Virulence genes and neutral DNA markers of Helicobacter pylori isolates from different ethnic communities of West Bengal, India. J Clin Microbiol. 2003;41:3737-43.
  5. Mohandas KM. Problems with epidemiologic studies on Helicobacter pylori in India. Indian J Gastroenterol. 1997;16:S6-8.
  6. Bhatia SJ, Bhandarkar PV, Aggarwal R, Sawant PD. Eradication of Helicobacter pylori in non-ulcer dyspepsia. Indian J Gastroenterol. 2000;19:S16-9.
  7. Chey WD, Vakil NB, Howden CW. Clinical rationale for confirmation testing after treatment of Helicobacter pylori infection: Implications of rising antibiotic resistance. Gastro-Hep. 2014;10:7-3.
  8. Fuccio L, Zagari RM, Eusebi LH, Laterza L, Cennamo V, Ceroni L et al. Meta-analysis: can Helicobacter pylori eradication treatment reduce the risk for gastric cancer? Ann Intern Med. 2009;151:121-8.
  9. Patel SK, Pratap CB, Jain AK, Gulati AK, Nath G. Diagnosis of Helicobacter pylori: What should be the gold standard? World J Gastroenterol. 2014;20:12847-59.
  10. Clayton C, Kleanthous K, Tabaqchali S. Detection and identification of Helicobacter pylori by the polymerase chain reaction. J Clin Pathol. 1991;44:515-6.
  11. De Reuse H, Labigne A, Mengin-Lecreulx D. The Helicobacter pylori ureC gene codes for a phosphoglucosamine mutase. J Bacteriol. 1997;179:3488-93.
  12. Ho SA, Hoyle JA, Lewis FA, Secker AD, Cross D, Mapstone NP et al. Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. J Clin Microbiol. 1991;29:2543-49.
  13. Hoshina S, Kahn SM, Jiang W, Green PH, Neu HC, Chin N et al. Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies. Diagn Microbiol Infect Dis. 1990;13:473-9.
  14. Rimbara E, Sasatsu M, Graham DY. PCR detection of Helicobacter pylori in clinical samples. Methods Mol Biol. 2013;943:279-87.
  15. Maeda S, Yoshida H, Ogura K, Kanai F, Shiratori Y, Omata M. Helicobacter pylori specific nested PCR assay for the detection of 23S rRNA mutation associated with clarithromycin resistance. Gut.1998;43:317-21.
  16. Singh V, Mishra S, Rao GR, Jain AK, Dixit VK, Gulati AK et al. Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60. Helicobacter. 2008;13:30-34.
  17. Mishra S, Singh V, Rao GR, Dixit VK, Gulati AK, Nath G. Prevalence of Helicobacter pylori in asymptomatic subjects—a nested PCR based study. Infect Genet Evol. 2008;8:815-9.
  18. Monteiro L, Cabrita J, Mégraud F. Evaluation of performances of three DNA enzyme immunoassays for detection of Helicobacter pylori PCR products from biopsy specimens. J Clin Microbiol. 1997;35:2931-6.
  19. Lage AP, Fauconnier A, Burette A, Glupczynski Y, Bollen A, Godfroid E. Rapid colorimetric hybridization assay for detecting amplified Helicobacter pylori DNA in gastric biopsy specimens. J Clin Microbiol. 1996;34:530-33.
  20. Van Doorn LJ, Figueiredo C, Rossau R, Jannes G, van As- broek M, Sousa JC et al. Typing of Helicobacter pylori vacA gene and detection of cagA gene by PCR and reverse hybridization. J Clin Microbiol. 1998;36:1271-6.
  21. El-Zaatari FA, Nguyen AM, Genta RM, Klein PD, Graham DY. Determination of Helicobacter pylori status by reverse transcription-polymerase chain reaction. Comparison with urea breath test. Dig Dis Sci. 1995;40:109-13.
  22. Oksanen K, Kainulainen H, Ruuska T, Mäki M, Ashorn M. Reverse transcription-polymerase chain reaction in the diagnosis of Helicobacter pylori infection in Finnish children. J Pediatr Gastroenterol Nutr. 1999;28:252-6.
  23. EUCAST. Breakpoint tables for interpretation of MICs and zone diameters. 2013; Version 3.1. Available from http://www.eucast. org/fileadmin/src/media/PDFs/ EUCAST files/Consultation/ EUCASTclinical breakpoints for Helicobacter pylori.pdf.
  24. Smith SM, O'Morain C, McNamara D. Antimicrobial susceptibility testing for Helicobacter pylori in times of increasing antibiotic resistance. World J Gastroenterol. 2014;20:9912-21.
  25. Agudo S, Alarcón T, Urruzuno P, Martínez MJ, López-Brea M. Detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies of pediatric patients by using a commercially available real-time polymerase chain reaction after NucliSens semiautomated DNA extraction. Diagn Microbiol Infect Dis. 2010;67:213-9.
  26. Lottspeich C, Schwarzer A, Panthel K, Koletzko S, Rüss- mann H. Evaluation of the novel Helicobacter pylori ClariRes real-time PCR assay for detection and clarithromycin susceptibility testing of H. pylori in stool specimens from symptomatic children. J Clin Microbiol. 2007;45:1718-22.
  27. Lehours P, Siffré E, Mégraud F. DPO multiplex PCR as an alternative to culture and susceptibility testing to detect Helicobacter pylori and its resistance to clarithromycin. BMC Gastroenterol. 2011;11:112.
  28. Cambau E, Allerheiligen V, Coulon C, Corbel C, Lascols C, Deforges L et al. Evaluation of a new test, genotype HelicoDR, for molecular detection of antibiotic resistance in Helicobacter pylori. J Clin Microbiol. 2009;47:3600-07.
  29. Miendje Deyi VY, Burette A, Bentatou Z, Maaroufi Y, Bon- tems P, Lepage P et al. Practical use of GenoType® HelicoDR, a molecular test for Helicobacter pylori detection and susceptibility testing. Diagn Microbiol Infect Dis. 2011;70:557-60.
  30. Harris A, Misiewicz JJ. Management of Helicobacter pylori infection. BMJ. 2001;323:1047-50.
  31. Caliskan R, Tokman HB, Erzin Y, Saribas S, Yuksel P, Bolek BK et al. Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey Revista da Sociedade Brasileira de Medicina Tropical. 2015;48:278-84.
  32. Datta S, Chattopadhyay S, Patra R, De R, Ramamurthy T, Hembram J et al. Most Helicobacter pylori strains of Kolkata in India are resistant to metronidazole but susceptible to other drugs commonly used for eradication and ulcer therapy. Aliment Pharmacol Ther. 2005;22:51-7.
  33. Thyagarajan SP, Ray P, Das BK, Ayyagari A, Khan AA, Dharmalingam S et al. Geographical difference in antimicrobial resistance pattern of Helicobacter pylori clinical isolates from Indian patients: Multicentric study. J Gastroenterol Hepatol. 2003;18:1373-78.
  34. Pandya HB, Agravat HH, Patel JS, Sodagar NRK. Emerging antimicrobial resistance pattern of Helicobacter pylori in central Gujarat. Indian J Med Microbiol. 2014;32:408-13.
  35. Mono R, Giorgio F, Carmine P, Soleo L, Cinquepalmi V, Ierardi E. Helicobacter pylori clarithromycin resistance detected by Etest and TaqMan real-time polymerase chain reaction: a comparative study. APMIS. 2012;120:712-7.
  36. McMahon BJ, Bruce MG, Koch A, Goodman KJ, Tsukanov V, Mulvad G et al. The diagnosis and treatment of Helicobacter pylori infection in Arctic regions with a high prevalence of infection: Expert Commentary. Epidemiol. Infect. 2016;144:225-33.
  37. Saha DR. Correlation of histology with genotypes of Helicobacter pylori isolated from cases of peptic ulcer, non- ulcer dyspepsia, gastric carcinoma and lymphoma. National Institute of Cholera And Enteric Diseases (NICED) Annual Report 2004-2005.
  38. Megraud F, Coenen S, Versporten A, Kist M, Lopez-Brea M, Hirschl AM et al. Study Group participants: Helicobacter pylori resistance to antibiotics in Europe and its relationship to antibiotic consumption. Gut. 2013;62:34-42.
  39. Cuadrado-Lavín A, Salcines-Caviedes JR, Carrascosa MF, Mellado P, Monteagudo I, Llorca J. et al. Antimicrobial susceptibility of Helicobacter pylori to six antibiotics currently used in Spain. J Antimicrob Chemother. 2012;67:170-73.
  40. O'Connor A, Gisbert J, O'Morain C. Treatment of Helicobacter pylori infection. Helicobacter. 2009;14:46-51.
  41. Glocker E, Stueger HP, Kist M. Quinolone resistance in Helicobacter pylori isolates in Germany. Antimicrob Agents Chemother. 2007;51:346-49.
  42. Larsen AL, Ragnhildstveit E, Moayeri B, Eliassen L, Melby KK. Resistance rates of metronidazole and other antibacterials in Helicobacter pylori from previously untreated patients in Norway. APMIS. 2013;121:353-8.
  43. Wueppenhorst N, Stueger HP, Kist M, Glocker EO. High secondary resistance to quinolones in German Helicobacter pylori clinical isolates. J. Antimicrob Chemother. 2013;68:1562-6.
  44. Miftahussurur M, Yamaoka Y. Appropriate First-Line Regimens to Combat Helicobacter pylori Antibiotic Resistance: An Asian Perspective. Molecules. 2015;20:6068-92.
  45. Boyanova L, Koumanov R, Gergova G, Popova M, Mitov I, Kovacheva Y et al. Prevalence of resistant Helicobacter pylori isolates in Bulgarian children. J. Med. Microbiol. 2002;51:786-90.
  46. Toro C, Garcia Samaniego J, Carbo J, Iniguez A, Alarcon T, Lopez-brea M et al. Prevalence of primary Helicobacter pylori resistance to eight antimicrobial agents in a hospital in Madrid. Rev. Esp. Quimioter. 2001;14:172-6.
  47. Mhasker M, Sandhu N, Abraham P. In vitro antimicrobial susceptibility of Helicobacter pylori strain in Indian patients. Indian J. Gastroenterol. 1997;16 (Suppl.): S35.
  48. Abraham P, Sandhu N, Naik SR. In vitro sensitivity of Helicobacter pylori in India. Indian J. Gastroenterol. 1997;16:S20-1.
  49. Sharma S, Prasad KN, Chamoli D, Ayyagari A. Antimicrobial susceptibility pattern and biotyping of Helicobater pylori isolates from patients with peptic ulcer diseases. Indian J. Med. Res.1995;102:261-6.
  50. Vasquez A, Valdez Y, Gilman RH, McDonald JJ, Westblom TU, Berg D et al. Metronidazole and Clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in colour indicator egg yolk agar in a miniwell format. J. Clin. Microbiol. 1996;34:1232-4.
  51. Piccolomini R, Bonaventura GD, Catamo G, Carbone F, Neri M. Comparative evaluation of the E-test, agar dilution and broth microdilution for testing susceptibilities of Helicobacter pylori strains to 20 antimicrobial agents. J. Clin. Microbiol. 1997;35:1842-6.
  52. Boyanova L, Mentis A, Gubina M, Rozynek E, Gosciniak G, Kalenic S et al. The status of antimicrobial reistance of Helicobacter pylori in eastern Europe. Clin Microbiol Infect. 2002;8:388-96.
  53. Hosseini V, Mokhtare M, Gholami M, Taghvaei T, Maleki I, Valizadeh M et al. A Comparison between Moderate- and Highdose Furazolidone in Triple Regimens for Helicobacter pylori Eradication in Iran. Middle East J Dig Dis. 2014;6:195-202.
  54. Ford AC, Axon AT. Epidemiology of Helicobacter pylori infection and public health implications. Helicobacter. 2010;15:S1-6.
  55. Parkinson AJ, Bruce MG, Zulz T. International circumpolar international surveillance, an Arctic network for surveillance of infectious diseases. Emerg Infect Dis. 2008;14:18-24.
  56. Szajewska H, Horvath A, Piwowarczyk A. Meta-analysis: the effects of Saccharomyces boulardii supplementation on Helicobacter pylori eradication rates and side effects during treatment. Aliment Pharmacol Ther. 2010;32:1069-79.
  57. Zou J, Dong J, Yu X. Meta-analysis: Lactobacillus containing quadruple therapy versus standard triple first-line therapy for Helicobacter pylori eradication. Helicobacter. 2009;14:97-107.
  58. Papastergiou V, Georgopoulos SD, Karatapanis S. Treatment of Helicobacter pylori infection: Past, present and future. World J Gastrointest Pathophysiol. 2014;5:392-9.
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