Lamellar keratoplasty was first performed by ophthalmic surgeons in the late 1800s. An LKP refers to the surgical procedure in which the anterior corneal layers are excised and replaced with donor corneal tissue. Classically, both the donor and recipient corneas are manually dissected, ideally to identical depths. Thus, the thickness of removed diseased corneal tissue will exactly equal to that of the donated corneal lenticule. This dissection can be performed with a blunt steel instrument, gas, fluid, or viscoelastic substance. Donor corneal lenticules were initially harvested from whole globes. Modern-day eye banks have moved from supplying whole globes to providing corneoscleral rims as corneal donor tissue. Although whole globes can be specifically requested, technicians are most often trained to prepare corneo­scleral rims rather than whole globes which require a more complex harvesting procedure. However, manual lamellar dissection of corneoscleral rims is technically more challenging than dissection performed on a whole globe. This has led to the development of various instruments that aid in supporting the corneoscleral rim during lamellar harvesting. Lamellar keratoplasty (LKP) has recently emerged to the corneal forefront as a surgical technique of significant interest to corneal surgeons. Improved surgical techniques have evolved as a result of advancement in technology and instrumentation. Preparation of donor lenticule is an integral part of LKP and this chapter focuses on the use of mechanical and femtosecond laser micro­keratomes and an artificial anterior chamber for harvesting donor corneal lenticules for LKP.