Deep lamellar endothelial keratoplasty (DLEK) is a new and evolving corneal lamellar surgery that has gained significant interest among corneal surgeons globally. The DLEK seems to be promising in the visual rehabilitation of patients with primary endothelial failure and secondary corneal edema. DLEK may be called no-stitch or sutureless corneal transplantation surgery since there are no sutures on the recipient cornea. DLEK may replace conventional penetrating keratoplasty (PKP) in selected cases of corneal endothelial decompensation. The author first reported the use of indocyanine green (ICG) within the human cornea, namely in DLEK. This chapter describes the technique of using of ICG on the stromal side of the donor corneal disk in DLEK, before placement of the corneal disk into the host anterior chamber. This quick staining method effectively localizes the donor disk in the host anterior chamber at all times during surgery, increases the accuracy of proper placement of this disk into the opening in the host corneal stroma, and helps in the alignment of the donor-host corneal stroma and endothelium. See chapters on DLEK for the surgical techniques, anesthesia, pre- and postoperative medications and follow-up. This chapter will describe the relevant surgical steps as it applies to the use of ICG in DLEK surgery. The author’s report is the first report of the use of ICG in DLEK surgery and it is also the first use of ICG intrastromally in the human cornea. The ICG usage in the manner described in this chapter seems to be safe and there have been no clinically observable adverse side effects from its use. The ICG appears to be useful in DLEK and may greatly facilitate the DLEK procedure for all cornea surgeons performing this procedure.