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Chapter-06 RBC Osmotic Fragility Test

BOOK TITLE: Laboratory Procedures in Haematology

Author
1. Mehdi SR
ISBN
9788180616570
DOI
10.5005/jp/books/10437_6
Edition
1/e
Publishing Year
2006
Pages
2
Author Affiliations
1. JN Medical College, AMU, Aligarh, India, Era’s Lucknow Medical College, Lucknow, Uttar Pradesh, India
Chapter keywords

Abstract

Osmotic Fragility test is carried out for the diagnosis of Hereditary Spherocytosis. The test is based on the principle that spherocytes are less resistant to hypotonic solutions than normal or hypochromic cells. Spherocytes are more fragile and easily hemolyse in hypotonic solutions. The procedure described here deals with the preparation of different concentrations of hypotonic solutions and treatment of suspected HS cases in comparison with normal RBC. The plotting of the curve depicts the result.

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