Protocols on storage and disinfection of laparoscopic instruments in an Indian hospital, like keeping them in gluteraldehyde solution, are given. Cleaning of instruments with detergents, enzymes, and the quality of water and brushes are described. Manual cleaning, Ultrasonic cleaning, Machine cleaning are described I detail. Use of chemicals for high level disinfection, like Glutaraldehyde, Orthophthaldehyde, and Peracetic acid are described. Methods of Steam sterilization, gas sterilization, Low-temperature steam with formaldehyde, gas plasma sterilization, and chemical sterilization are described. Methods of testing for sterilization and disinfection are given. Tips for checking for damage to instruments like insulation inspection, checking of jaws and joints, sealing and contact surfaces are described.