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by P Ramadass, A Wilson Aruni
This book compiles practical applications and protocols in areas of applied biotechnology and molecular biology. The topics discussed in detail include cell culture, hybridoma technology, nucleic acid isolation and electrophoresis, nucleic acid probes and hybridization methods, PCR and related techniques, and nucleic acid sequencing methods. This comprehensive textbook elaborates all the above topics in detail, highlighting the principles, and step-by-step protocols. This practical book also provides the desired knowledge to students interested in studying different molecular methods and their vast applicability in various fields of science and technology. The book is helpful in finding the substantial role of practical biotechnology in the development of the health care and chemical industries by the availability of several diagnostic, prophylactic and therapeutic products.
|Chapter-01 Cell Culture||1-38|
|Chapter-02 Monoclonal Antibody Production||39-51|
|Chapter-03 DNA and RNA Isolation and Electrophoresis||52-86|
|Chapter-04 Methods for Labeling Nucleic Acids||87-126|
|Chapter-05 Gene Cloning||127-164|
|Chapter-06 Gene Sequencing||165-185|
|Chapter-07 Polymerase Chain Reaction||186-197|
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