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Chapter-24 Estimation of serum bilirubin by Malloy-Evelyn method

BOOK TITLE: Laboratory Manual for Practical Biochemistry

Author

1. MK Ganesh
2. A Hemavathi
3. YM Shivaraja Shankara

ISBN

9788184482591

DOI

10.5005/jp/books/10435_25

Edition

1/e

Publishing Year

2008

Pages

Author Affiliations

1. KVG Dental College, Sullia, Karnataka, KVG Dental College, Sullia, Karnataka, India
2. KVG Medical College, Sullia, Karnataka
3. KVG Medical College, Sullia, Karnataka, KVG Medical College, Sullia, Karnataka, India

Chapter keywords

Abstract

Abstract

Bilirubin is a product of heme catabolism, made water soluble by conjugation in liver and secreted into bile forming urobilinogens in intestine. Serum bilirubin estimation methods are based on Van den Bergh reaction in which bilirubin forms azobilirubin on treating with diazotized sulphanilic acid. Hyperbilirubinemia is manifested as jaundice. Serum bilirubin estimation is a test assessing the excretory function of liver and useful in differential diagnosis of jaundice.

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