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Chapter-26 Estimation of urine creatinine by Jaffe's alkaline picrate method

BOOK TITLE: Laboratory Manual for Practical Biochemistry

Author
1. MK Ganesh
2. A Hemavathi
3. YM Shivaraja Shankara
ISBN
9788184482591
DOI
10.5005/jp/books/10435_27
Edition
1/e
Publishing Year
2008
Pages
3
Author Affiliations
1. KVG Dental College, Sullia, Karnataka, KVG Dental College, Sullia, Karnataka, India
2. KVG Medical College, Sullia, Karnataka
3. KVG Medical College, Sullia, Karnataka, KVG Medical College, Sullia, Karnataka, India
Chapter keywords

Abstract

Creatinine in urine is determined by its reaction with picric acid in alkaline medium to form orange colored tautomer of creatinine picrate (Jaffe’s reaction). The intensity of the orange color is read using green filter (540 nm). From the OD values, the concentration of creatinine per 100 ml and per day is calculated. Since creatinine of urine is high, it is suitably diluted.

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