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Chapter-03 Specimen Collection

BOOK TITLE: Arvind’s Atlas of Fungal Corneal Ulcers: Clinical Features and Laboratory Identification Methods

Author

1. Prajna N Venkatesh
2. Prajna Lalitha
3. Vijayakumar
4. Srinivasan M

ISBN

9788184483659

DOI

10.5005/jp/books/10057_3

Edition

1/e

Publishing Year

2008

Pages

Author Affiliations

1. Aravind Eye Hospital, Madurai, India, Aravind Eye Hospital, Madurai, Tamil Nadu, India, Aravind Eye Hospital, No.1, Anna Nagar, Madurai – 625 020, Aravind Eye Hospital and Postgraduate Institute, Madurai, Tamil Nadu, India, Aravind Eye Care System, Madurai, Tamil Nadu, India
2. Aravind Eye Hospital, Madurai, Tamil Nadu, India, Aravind Eye Hospital and Postgraduate Institute, Madurai, Tamil Nadu, India, Aravind Eye Hospital and Postgraduate, Institute of Ophthalmology, Madurai, Tamil Nadu, India
3. Aravind Eye Hospital, Madurai, Tamil Nadu, India

Chapter keywords

Abstract

Abstract

This chapter explains the procedure of obtaining material from the surface of the cornea from a patient with corneal ulcer which is performed under aseptic conditions by an ophthalmologist using a sterile Kimura spatula. The procedure is performed under magnification of a slit lamp or binocular loupe following instillation of topical anesthetic agents such as 0.5% proparacaine or 4% lignocaine. If the ulcer may be predominate in the deeper stromal lesion, with inflammatory outpourings in the anterior chamber, a paracentesis is made using a 26 gauge needle mounted on a 2cc plastic disposable tuberculin syringe. Sample collection procedures and materials required for sample collection are explained with colour photographs.

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