Classical methods such as cat spleen model, rat blood pressure, cat model of nictitating membrane prolapsed, pithed rat model, rat heart and uterus model are available to evaluate sympathomimetic and sympatholytic drugs. Utilizing tissue organ bath system, rabbit pulmonary artery, rat seminal vesicle, rat vas deferens, cat splenic strip, guinea pig tracheal chain, guinea pig isolated heart, rat aorta, rat submaxillary tissue etc. tissue preparations can be made to evaluate drugs acting on sympathetic nervous system.