EXPORT CITATION

Chapter-55 Molecular Diagnostics

BOOK TITLE: Textbook of Biochemistry for Medical Students

Author
1. Vasudevan DM
2. S Sreekumari
3. Vaidyanathan Kannan
ISBN
9789350250167
DOI
10.5005/jp/books/11359_55
Edition
6/e
Publishing Year
2011
Pages
13
Author Affiliations
1. Faculty of Medicine, Amrita Vishwa Vidyapeetham, (Amrita University), Kochi, Kerala, Formerly Principal, College of Medicine, Amrita, Kerala; Formerly, Dean, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, Amrita Vishwa Vidyapeetham (Deemed University), Cochin, Kerala, E-mail: dmvasudevan@aims.amrita.edu, PG Programs and Research College of Medicine, Amrita Institute of Medical Sciences, Kochi, Kerala, India, College of Medicine, Amrita Institute of Medical Sciences, Kochi, Kerala, India; Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India
2. Jubilee Mission Medical College, Trissur, Kerala, Sree Gokulam Medical College and Research Foundation, Thiruvananthapuram, Kerala, India, Jubilee Mission Medical College and Research Institute, Thrissur, Kerala, India, Government Medical College, Thrissur and Thiruvananthapuram, Kerala, India; Sree Gokulam Medical College and Research Foundation, Thiruvananthapuram, Kerala, India; Jubilee Mission Medical College and Research Institute, Thrissur, Kerala, India
3. Amrita Institute of Medical Sciences, Kochi, Kerala, India, Pushpagiri Institute of Medical Sciences and Research Centre, Thiruvalla, Kerala, India, Believers Church Medical College and Hospital, Thiruvalla, Kerala, India
Chapter keywords

Abstract

A probe is a single stranded piece of DNA, labeled with a radioactive or a non-radioactive label, the sequence of which of complementary to the target DNA. Southern blot is a technique used to detect a specific segment of a DNA. It has diagnostic and forensic applications. Northern blot is used to detect a specific RNA. Western blot is used to detect a specific protein. Molecular cloning in medicine can be used for diagnosis of genetic diseases such as Duchenne Muscular Dystrophy (DMD), Huntington’s chorea, Sickle cell anemia, etc. Polymerase chain reaction (PCR) is an in vitro DNA amplification procedure in which millions of copies of a particular sequence of DNA can be produced within hours. The enzyme used in PCR is TAQ polymerase Applications of PCR include diagnosis of bacterial and viral diseases, genetic disorders, cancers, solving medicolegal cases and in paleontology. Hybridoma technique is used to generate monoclonal antibodies. They are used in immunological research, ELISA, nephelometry, etc.

© 2019 Jaypee Brothers Medical Publishers (P) LTD.   |   All Rights Reserved