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Chapter-18 POLYMERASE CHAIN REACTION (PCR) AND REAL-TIME PCR (For Detection and Quantification of Nucleic Acid)

BOOK TITLE: Textbook of Medical Biochemistry

Author

1. Chatterjea MN
2. Shinde Rana

ISBN

9789350254844

DOI

10.5005/jp/books/11486_18

Edition

8/e

Publishing Year

2012

Pages

Author Affiliations

1. Armed Forces Medical College, Pune, Armed Forces Medical College, Pune, India, Armed Forces Medical College, Pune, Maharashtra, India
2. SSR Medical College, Belle Rive, Mauritius

Chapter keywords

Polymerase chain reaction, denaturation, annealing, extension, deoxynucleotide triphosphates, DNA polymerase, primer, thermostable DNA polymerase, viral load, reverse transcription-PCR, asymmetric PCR, inverse PCR, nested PCR, anchored PCR, gel electrophoresis, amplification, molecular beacons, fluorescence resonance energy transfer, fluorescent dye, relative quantitation, absolute quantitation, nylon membrane, biotin

Abstract

Abstract

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