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Chapter-29 Methods of Separation and Assay of Biological Compounds

BOOK TITLE: Textbook of Biochemistry for Dental Students

Author
1. Vasudevan DM
2. S Sreekumari
3. Vaidyanathan Kannan
ISBN
9789350254882
DOI
10.5005/jp/books/11333_29
Edition
2/e
Publishing Year
2011
Pages
7
Author Affiliations
1. Faculty of Medicine, Amrita Vishwa Vidyapeetham, (Amrita University), Kochi, Kerala, Formerly Principal, College of Medicine, Amrita, Kerala; Formerly, Dean, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, Amrita Vishwa Vidyapeetham (Deemed University), Cochin, Kerala, E-mail: dmvasudevan@aims.amrita.edu, PG Programs and Research College of Medicine, Amrita Institute of Medical Sciences, Kochi, Kerala, India, College of Medicine, Amrita Institute of Medical Sciences, Kochi, Kerala, India; Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India
2. Jubilee Mission Medical College, Trissur, Kerala, Sree Gokulam Medical College and Research Foundation, Thiruvananthapuram, Kerala, India, Jubilee Mission Medical College and Research Institute, Thrissur, Kerala, India, Government Medical College, Thrissur and Thiruvananthapuram, Kerala, India; Sree Gokulam Medical College and Research Foundation, Thiruvananthapuram, Kerala, India; Jubilee Mission Medical College and Research Institute, Thrissur, Kerala, India
3. Amrita Institute of Medical Sciences, Kochi, Kerala, India, Pushpagiri Institute of Medical Sciences and Research Centre, Thiruvalla, Kerala, India, Believers Church Medical College and Hospital, Thiruvalla, Kerala, India
Chapter keywords

Abstract

Electrophoresis refers to the movement of charged particles when subjected to an electrical field. Cellulose acetate electrophoresis is used for separation of lipoproteins and hemoglobin. Agar electrophoresis is used to separate serum proteins. Thin layer chromatography is widely used to separate and identify the amino acids. Southern blot technique is used to detect specific segment of DNA in the whole genome. Northern blot is to identify RNA. Western blot analysis is done to identify proteins. Radio-immunoassay is used to determine very minute quantities of substances in biological fluids. The specificity of antibody and the sensitivity of radioactivity are combined in RIA. Disadvantage of RIA is that it uses radio-activity and so detrimental to health. In enzyme linked immunosorbent assay (ELISA), horse radish peroxidase (HRP) is tagged to the enzyme.

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