Chapter-08 Histograms

BOOK TITLE: The ABC of CBC: Interpretation of Complete Blood Count and Histograms

1. Lokwani DP
Publishing Year
Author Affiliations
1. Madhya Pradesh Medical Sciences University, Jabalpur, Madhya Pradesh, India; Netaji Subhash Chandra Bose (NSCB) Medical College, Jabalpur, Madhya Pradesh, India; Diplomate National Board Courses, Jabalpur Hospital and Research Center, Jabalpur, Madhya Pradesh, India
Chapter keywords
Histograms, Platelet histogram, RBC histogram, Red cell flags, Volume histogram, WBC histogram


Interpretation of histograms and scatter plots, in conjunction with the numerical data can be clinically useful in the diagnosis and follow-up of many hematological and nonhematological conditions. The volume histogram reflects the size of erythrocytes or any other particle in the erythrocyte size range. Diagrammatic representation of histogram designed for better understanding of interpretation of hematological disorders, with the help of variations of histograms seen in CBC reports, for learners. The WBC histogram is generated with size of cells in femtoliters on the x-axis and the relative frequency of the cells on the y-axis. The automated counter sets a lower discriminator (LD) fluctuating between 30 and 60 fL and an upper discriminator (UD) fixed at 300 fL. The number of cells between the UD and the LD is the WBC count. WBC histograms consist of two troughs, T1 between 78 and 114 fL and T2 < 150 fL. Platelet derived histograms are obtained from volume sizes of 2 to 30 fL. Platelet counting and sizing in both the electrical impedance and optical systems reflect the native cell size. The counting of the platelets takes place in the RBC aperture and the instrument is designed to count the particles in a range of 0 to 70 fL.

© 2019 Jaypee Brothers Medical Publishers (P) LTD.   |   All Rights Reserved