This chapter provides detail description of Novel approaches to the diagnosis and treatment of Eustachian tube disorders. Abstract of the chapter is given in the beginning. Chapter has 9 well illustrated diagrams and pictures. Critical anatomical and physiological aspects of Eustachian tube, peritubal muscles and their functions, visual identification and landmarks of Eustachian tube, Eustachian tube dilatation and closure, middle ear gas exchange, clearance of the middle ear, protection of the middle ear, evaluation of the Eustachian tube, pathology of Eustachian tube dysfunction, medical treatment for Eustachian tube dilatory dysfunction, surgical treatment for Eustachian tube dilatory dysfunction and patulous Eustachian tube dysfunction are described in detail. 37 references are included at the end of the chapter to facilitate further reading.