Glaucoma is said to be leading cause of blindness worldwide. Symptom of dry eye is more prevalent in individuals with glaucoma. Various studies reporting the symptoms of glaucoma were tabulated. Use of antiglaucoma agents containing preservatives also increases the risk of ocular surface disease. Various preservatives used in ocular medicine are discussed. These preservatives inhibit the growth of infectious agents thus prolonging the shelf life and thereby maintain drug potency. Cellular and bio molecular changes in conjunctival and corneal epithelial cell that may be due to the use of preservatives are described. Various clinical studies to support these results are given. Adverse reaction of drugs, side effects of antiglaucoma treatment and other discomfort to the patient affect the success of treatment and also the compliance. Also, a formulation of timolol, only antiglaucoma agent without preservative is discussed. Various in vitro and in vivo studies are complied to show timolol non toxic nature. In addition, soft preservative, like sofZia is also discussed briefly.