Laboratory Manual for Practical Biochemistry

Shivaraja Shankara YM; Ganesh  MK; Shivashankara  AR

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Laboratory Manual for Practical Biochemistry

by Shivaraja Shankara YM, Ganesh MK, Shivashankara AR

This book fulfills the need for a comprehensive book on Practical Biochemistry. Practical Clinical Biochemistry in a nutshell. Questions are given at the end of chapters. It covers all the basic aspects of Practical Biochemistry such as basic equipments and glassware, basic calculations, laboratory hazards, preparation of molar and normal solutions and preparation of buffers. Basic qualitative and quantitative tests are explained. Advanced aspects such as chromatography, electrophoresis, quality control and automation are discussed. Reference ranges for all the routinely done Biochemical tests are given. Case-oriented approach: wherever applicable, clinical significance of tests done in clinical biochemistry laboratory is highlighted. Case reports are given, which helps the students to develop a clinical-oriented approach and integrates the basic Biochemistry with Clinical Medicine.

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Book Detail

Author	Author's Affiliation
1. YM Shivaraja Shankara	KVG Medical College, Sullia, Karnataka KVG Medical College, Sullia, Karnataka, India
2. MK Ganesh	KVG Dental College, Sullia, Karnataka KVG Dental College, Sullia, Karnataka, India
3. AR Shivashankara	Father Muller Medical College, Mangalore, Karnataka, India
ISBN	9789350902769
Speciality	Biochemistry
DOI	10.5005/jp/books/12009
Edition	2/e
Publishing Year	2013
Pages	180
Book Type	Textbook

Chapters

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	Chapter Name	Pages	Action
	Preliminary Pages	i-xviii
	Index	161-167
	Chapter-01 Laboratory Equipments, Glassware, Laboratory Hazards and General Laboratory Rules	1-10
	Chapter-02 Laboratory Units, Basic Calculations and Preparation of Solutions	11-14
	Chapter-03 Acids, Bases, Alkalies, pH, Indicators and Buffers	15-18
	Chapter-04 Carbohydrates	19-22
	Chapter-05 Reactions of Carbohydrates	23-29
	Chapter-06 Proteins	30-31
	Chapter-07 Color Reactions of Proteins	32-37
	Chapter-08 Precipitation Reactions of Proteins	38-42
	Chapter-09 Reactions of Non-protein Nitrogenous (NPN) Substances	43-44
	Chapter-10 Scheme for Identification of Physiologically Important Substances	45-45
	Chapter-11 Urine Analysis (Normal Urine)	46-50
	Chapter-12 Analysis of Abnormal Constituents in Urine	51-54
	Chapter-13 Inborn Errors of Metabolism	55-58
	Chapter-14 Analysis of Gastric Juice	59-61
	Chapter-15 Analysis of Saliva	62-63
	Chapter-16 Preparation of Hemin Crystals	64-64
	Chapter-17 Sample Collection and Preservation	65-68
	Chapter-18 Estimation of Blood Sugar by Folin-Wu Method	69-73
	Chapter-19 Estimation of Blood Glucose by O-toluidine Method	74-75
	Chapter-20 Estimation of Blood Urea by Diacetyl Monoxime (DAM) Method	76-78
	Chapter-21 Estimation of Serum Total Protiens, Albumin and Albumin/Globulin Ratio by Biuret Method	79-81
	Chapter-22 Estimation of Serum Albumin by Dye-binding Method	82-83
	Chapter-23 Estimation of Serum Inorganic Phosphate by Fiske and Subbarow Method	84-86
	Chapter-24 Estimation of Serum Cholesterol by Zak's Method	87-88
	Chapter-25 Estimation of Serum Uric Acid by Caraway Method	89-90
	Chapter-26 Estimation of Serum Bilirubin by Malloy and Evelyn Method	91-93
	Chapter-27 Estimation of Serum Creatinine by Jaffe's Alkaline Picrate Method	94-95
	Chapter-28 Estimation of Vitamin C (Ascorbic Acid) in Plasma by Sauberlich Method	96-97
	Chapter-29 Estimation of Vitamin A in Serum by Bessey Method	98-98
	Chapter-30 Estimation of Serum Transaminases by Reitman and Frankel Method	99-101
	Chapter-31 Estimation of Serum Alkaline Phosphatase by Kind and King Method	102-103
	Chapter-32 Estimation of Serum Calcium by O-cresolphthalein Complexone Method	104-105
	Chapter-33 Estimation of Urine Creatinine by Jaffe's Alkaline Picrate Method	106-107
	Chapter-34 Estimation of Urinary Proteins by Turbidimetric Method	108-110
	Chapter-35 Colorimetry—General Principle and Procedure	111-115
	Chapter-36 Potentiometry	116-119
	Chapter-37 Flame Photometer—General Principle and Procedure	120-122
	Chapter-38 Electrophoresis—General Principle and Procedure	123-125
	Chapter-39 Chromatography—General Principle and Procedure	126-129
	Chapter-40 Automation in Clinical Biochemistry	130-131
	Chapter-41 Quality Control in Clinical Biochemistry	132-135
	Chapter-42 Oral Glucose Tolerance Test	136-140
	Chapter-43 Case Reports	141-147
	Chapter-44 Normal Values of Biochemical Parameters	148-152
	Chapter-45 Reagent Preparations	153-160