Chapter-24 Western Blot Tests for Detection of Antibody to Extractable Nuclear Antigen
BOOK TITLE: Manual of Immunopathological Techniques
Author
1. Agrawal Amita
ISBN
9789351520849
DOI
10.5005/jp/books/12385_24
Edition
1/e
Publishing Year
2014
Pages
6
Author Affiliations
1.
Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India
Chapter keywords
Western blot,
Extractable nuclear antigen (ENA),
SDS-PAGE,
Nitrocellulose membrane,
Rabbit thymus,
Autoimmune diseases,
Acrylamide,
Bisacrylamide,
TEMED,
Whatman’s paper,
Separating gel,
Stacking gel,
Tris-buffer saline,
Blocking buffer,
Ponceau’s staining solution,
Comassi brilliant blue (CBB)