With excellent soft tissue and contrast resolution, magnetic resonance imaging (MRI) evolved remarkably for pituitary gland imaging. The imaging armamentarium consists of radiographs that include: computed tomography (CT), MRI, inferior petrosal sinus sampling using digital subtraction angiography (DSA), and positron emission tomography computed tomography (PET CT). BY using different imaging techniques, the anatomy of pituitary gland is described briefly. The indications for the imaging are: hyperfunctioning gland, hypofunctioning gland, and diabetes insipidus (DI). The detailed description of these three indications is given in this chapter. Different modalities gives high resolution images for optimal interpretation and based on these images the diagnosis can be different by correlating with the clinical features.