Cryotherapy is a controlled and targeted destruction of diseased tissue by application of a cold substance. It is used for the treatment of diverse benign lesions and well-circumscribed premalignant and malignant tumors. It is a very safe, inexpensive, reproducible, repeatable and simple office procedure. It requires short preparation time, is a sutureless procedure with minimal risk of infection and no anesthesia is usually required. It can be performed at any age including elderly patients with pacemakers in whom electrocautery is contraindicated, those on anticoagulants, patients allergic to anesthetic agents, patients with transmissible conditions such as HIV and hepatitis, during pregnancy and over most of the body sites. It provides high healing rates even in difficult areas with excellent cosmetic results if performed appropriately. This chapter also provides appropriate detail about the history of cryotherapy, mechanism of cryoablation, cryotherapy facility, cryosurgery equipments, methodology of cryosurgery, preoperative preparation and treatment. Post-treatment care, indications of cryotherapy, complications and contraindications to cryotherapy are also discussed in this chapter.