The genetic integrity of the spermatozoan is essential for normal embryo development. A high level of DNA fragmentation in sperm cells may represent a cause of male infertility that conventional examinations—sperm concentration, motility analysis, morphology assessment—cannot detect. The principle is based on interpreting the difference between fragmented and intact DNA in spermatozoa. The spermatozoa embedded in agarose matrix are overlaid on the coated slide and exposed to acid solution followed by lysis solution. After exposure, the spermatozoa with nonfragmented DNA shows the dispersed halos of DNA while the spermatozoa with fragmented DNA shows minimal or no halos. Fresh, raw, diluted, frozen or thawed and processed semen can be analyzed by this kit. Moreover, the test is easy to interpret, fast, reliable, reproducible and equivalent to sperm chromatin structure assay.