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Chapter-09 Different Semen Washing Techniques

BOOK TITLE: ISARCON 2017 Manual of Data Management and Quality Control, March to Perfection Complete Hands of IVF

Author
1. Joshi Pratixa
ISBN
9789352700790
DOI
10.5005/jp/books/14173_10
Edition
1/e
Publishing Year
2018
Pages
5
Author Affiliations
Chapter keywords
Assisted reproductive technique, ART, semen sample, semen preparation method, density gradient centrifugation, spermatozoa, oligozoospermia, teratozoospermia, asthenozoospermia, semen preparation, ejaculate, sperm processing technique, sperm separation method, swim-up method, two-layer discontinuous gradient centrifugation, pentoxifylline wash, test-yolk buffer method, sedimentation method, polyvinylpyrrolidone droplet swim-out method

Abstract

The application of assisted reproductive techniques (ART) has revolutionized the treatment of infertility. With ART, semen samples must first be processed before they can be used for insemination. Sperm preparation methods seek to replicate in vitro the natural process in which viable sperm are separated from other constituents of the ejaculate as they actively migrate through the cervical mucus. An ideal sperm processing technique should be gentle and one that recovers a highly functional sperm. Various sperm separation or isolation methods include swim-up methods, two-layer discontinuous gradient centrifugation, pentoxifylline wash, test-yolk buffer, sedimentation methods, polyvinylpyrrolidone (PVP) droplet swim-out, electrophoresis and fluorescence cell sorting methods. Retrograde ejaculation occurs when semen is directed into the urinary bladder during ejaculation.

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