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Chapter-33 Reticulocyte Count

BOOK TITLE: Textbook of Pathology for Allied Health Sciences

Author
1. Nayak Ramadas
ISBN
9789352701070
DOI
10.5005/jp/books/14184_34
Edition
1/e
Publishing Year
2017
Pages
2
Author Affiliations
1. Kasturba Medical College, Mangalore, Manipal University, Karnataka, India, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India, Yenepoya Medical College, Constituent of Yenepoya University (Accredited by NAAC with "A" grade), Mangaluru, Karnataka, India, Yenepoya Medical College, Yenepoya University, Mangaluru, Karnataka, India, Yenepoya Medical College, Yenepoya University (Accredited by NAAC with “A” grade), Mangaluru, Karnataka, India
Chapter keywords
Reticulocyte count, red blood cell, RBC, bone marrow, ribosome, visual method, automated method, RNA

Abstract

The objective of this chapter is to introduce the reticulocyte count. Reticulocytes are immature, non-nucleated RBCs released from bone marrow. They are slightly larger than the mature RBCs. They continue to synthesize hemoglobin after loss of the nucleus. Methods of reticulocyte count are visual method and automated method. Staining in living state is known as supravital staining. Reticulocytes contain ribosomes and RNA, which can be stained by supravital stains like brilliant cresyl blue and new methylene blue in the live and unfixed state. Reticulocyte count can also be performed in automated cell counters or by a flow cytometer. It is superior and accurate to manual count.

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