This chapter describes cultural characteristics of bacterial growth in liquid media and solid media. Bacterial growth on a culture plate and in a liquid culture medium will be provided to student for identification of bacterial culture. Liquid medium is prepared from the same culture plate which is given for examination and has the same bacteria. Therefore, first identify the liquid culture medium and describe the characteristics of growth. Hanging drop preparation is made from this liquid media for motility and morphology of the bacteria. Hanging drop preparation is done to demonstrate motility and to study morphology of bacteria. It is prepared from the bacterial growth provided in liquid culture medium in a test tube. A provisional diagnosis can be made on the basis of culture media and liquid medium findings. Further tests such as fermentation and other biochemical reactions, serology, etc. should be used to confirm the identification of bacteria.