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Chapter-07 Bacterial Culture Identification—A Specific Approach

BOOK TITLE: Practical Microbiology for MBBS Students

Author
1. Kumar Surinder
ISBN
9789352701902
DOI
10.5005/jp/books/14195_8
Edition
1/e
Publishing Year
2018
Pages
34
Author Affiliations
1. Maulana Azad Medical College, New Delhi, India
Chapter keywords
Solid media, nutrient agar, blood agar, MacConkey agar, bacterial growth, liquid media, Gram staining, colonial morphology, biochemical test, presumptive diagnosis, confirmatory diagnosis

Abstract

This chapter seeks to familiarize the readers with the identification of solid media such as nutrient agar, blood agar and MacConkey agar and bacterial growth on these media. A detailed discussion on scheme for identification of bacterial growth on solid and in liquid media is also provided. Characteristic features in Gram staining from culture, colonial morphology of an organism from solid media are also defined in this chapter. Appropriate biochemical tests for presumptive and confirmatory diagnosis are also described. Important questions related to Pseudomonas Aeruginosa, Staphylococcus Aureus, Streptococcus spp., Proteus spp., Escherichia Coli, Klebsiella spp., Shigella spp., and salmonellae are also given in this chapter along with their crisp and to the point answers.

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