The chapter focuses on platelet-rich plasma (PRP) which is a plasma rich in growth factors. It aims to give comprehensive perspective of usage of PRP, methodology of preparation and their limitations. PRP is a volume of the plasma fraction of autologous blood where platelet concentration is usually more than 10,00,000 platelets/μL from the baseline. Platelets are natural sources of growth factors and play a fundamental role in hemostasis. Preparation of platelet-rich plasma is a day care process performed under strict aseptic conditions as well as under optimum temperature regulations, i.e. 20–22°C after taking patient consent. In order to inhibit platelet aggregation, it is prepared with an anticoagulant, commonly using anticoagulant citrate dextrose solution formula A (ACD-A) or sodium citrate. Centrifugation separates blood components due to their varying specific gravities, red blood cells—heaviest, followed by white blood cells, platelets—lightest. Techniques of preparation are Vacutainer technique, PRP method or buffy coat method, using commercially available PRP kits, and modified syringe technique. Other topics discussed are activation of platelets, techniques of PRP delivery, classification of platelet-rich plasma, indications of PRP in dermatology and esthetics, and contraindications.