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Chapter-12 Principles of Colorimetry

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_13
Edition
2/e
Publishing Year
2018
Pages
8
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Colorimetry, photometry, colorimeter, absorbance, transmittance, plasma, serum, optical density, OD, Lambert’s law, Beer’s law

Abstract

Many biochemical experiments involve the measurement of a compound present in a complex mixture. The most widely used method for determining the concentration of biochemical compounds is colorimetry. This chapter covers the photometry, colorimeter, preparation of solution for investigation, technique, relationship between absorbance and transmittance, selection of filter in colorimetric estimation, and application of colorimeter. Colorimetric procedure is widely used in laboratories for the estimation of various biochemical compounds in various biological samples like blood, plasma, serum, CSF, urine and other body fluids.

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