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Chapter-13 Estimation of Blood Sugar

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_14
Edition
2/e
Publishing Year
2018
Pages
7
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Blood sugar, blood glucose, diabetes mellitus, Folin-Wu’s method, glucose oxidase method, O-toluidine method, Nelson-Somogyi method

Abstract

The main sugar of blood is glucose along with other carbohydrate constituents. Hence, ‘blood glucose’ is commonly referred as ‘blood sugar’. Blood glucose estimation is a common test done in all laboratories because it helps in diagnosis, management of diabetes mellitus and is a common prerequisite for any surgery. This chapter covers the methods of estimation, choice of blood specimen, preservation of blood, estimation of blood sugar by Folin-Wu’s method, glucose oxidase method, reagents, and procedure. Glucose oxidase (GOD) acts on glucose to produce gluconic acid hydrogen peroxide. Hydrogen peroxide is producing nascent oxygen by preoxidase (POD). Nascent oxygen further reacts with a chromogen to produce colored product, which is estimated colorimetrically.

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