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Chapter-14 Estimation of Blood Urea

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_15
Edition
2/e
Publishing Year
2018
Pages
4
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Blood urea, liver disease, kidney disease, diacetyl monoxime, DAM, nephrotic syndrome, chronic renal failure, malignant hypertension

Abstract

Urea is the metabolic end product derived from the catabolism of proteins. It is synthesized in the liver from ammonia and carbon dioxide and is excreted by kidney. Urea reacts with diacetly monoxime under strong acidic condition in presence of ferric ions and thiosemicarbazide to give a pink colored complex. Intensity of color is a measure of amount of urea present in blood. Color intensity is compared with standard and is measured using green filter (540 nm). Blood urea level decreases in liver diseases due to decreased synthesis. Blood urea level is commonly monitored to evaluate kidney diseases.

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