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Chapter-16 Estimation of Serum Inorganic Phosphate

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_17
Edition
2/e
Publishing Year
2018
Pages
5
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Serum inorganic phosphate, soft tissue, coenzyme, calcium, magnesium, potassium, sodium

Abstract

Phosphorus in the body is mainly present in the bones; small portion is present in cells and soft tissue. It is a component of many important biological compounds, e.g. some proteins, lipids, nucleic acid and coenzymes. It plays an important role in acid–base regulation particularly by the kidneys. The serum phosphate may exist as free ions (40%) or in a complex form (50%) with cations, such as calcium, magnesium, sodium, potassium, etc. About 10% of serum phosphate is bound to proteins. A quantitative method for the estimation of inorganic phosphate was first developed by Fiske and Subbarow.

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