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Chapter-17 Estimation of Serum Total Proteins

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_18
Edition
2/e
Publishing Year
2018
Pages
9
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Serum, albumin, globulin, conjugated protein, serum mucoid, lipoprotein, biuret method, autoanalyzer method, color development

Abstract

Serum proteins represent a complex mixture containing a number of components which differ in properties and functions. Major components of serum proteins are albumin, globulins, and conjugated proteins. The peptide bonds (–CO–NH) present in the protein react with copper sulfate in an alkaline medium to form a purple/violet colored complex. The intensity of this color is proportional to the number of peptide linkages present and thus is a measure of the concentration of proteins.

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