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Chapter-19 Electrophoresis

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_20
Edition
2/e
Publishing Year
2018
Pages
6
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Electrophoresis, serum protein, Paper electrophoresis, Agarose gel electrophoresis, lipoprotein, nephrotic syndrome, hypoalbuminemia, chronic infection, charged particle

Abstract

Electrophoresis is a popular technique used to separate closely related compounds. It is based on the movement of charged particles in the electric field. This technique is based on the principle that positively charged particles migrate toward cathode and negatively charged particles migrate toward the anode. Rate of migration depends on the charge of the molecule. Application of electrophoresis, factors affecting migration of charged particles, types of electrophoresis, basic requirements of electrophoresis, paper electrophoresis, separation of plasma proteins, and movement of different protein fractions are covered in this chapter.

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