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Chapter-21 Estimation of Serum Aspartate Aminotransferase and Alanine Aminotransferase

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_22
Edition
2/e
Publishing Year
2018
Pages
4
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Alanine aminotransferase, ALT, aspartate aminotransferase, AST, cardiac disease, serum aspartate aminotransferase, enzyme, dinitrophenylhydrazine, DNPH

Abstract

Human serum contains several transaminases of which alanine aminotransferase (ALT) (SGPT) and aspartate aminotransferase (AST) (SGOT) are of diagnostic significance. For estimation of AST serum is incubated with aspartate and alpha ketoglutarate in phosphate buffer (pH 7.4) for 60 minutes. In the estimation of ALT, serum is incubated with alanine and alpha ketoglutarate for 30 minutes. After a measured time the reaction is stopped. Oxaloacetate is formed from AST and is spontaneously decarboxylated to pyruvate. Aspartate aminotransferase is increased in cardiac diseases.

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