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Chapter-23 Estimation of Plasma Ascorbic Acid

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_24
Edition
2/e
Publishing Year
2018
Pages
2
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Plasma ascorbic acid, acid solution, vitamin C, trichloroacetic acid, 2, 6-dichlorophenolindophenol, dehydroascorbic acid, metaphosphoric acid

Abstract

The aim of this chapter is to focus on the estimation of plasma ascorbic acid. The protein free filtrate is titrated with the dye 2,6 dichlorophenolindophenol in acid solution. The blue compound is red in acid solution, and on titration with a solution of ascorbic acid, is reduced to a colorless leuco base, the ascorbic acid being oxidized to dehydroascorbic acid. Plasma values are of limited value in diagnosing scurvy and subclinical conditions of vitamin C undernutrition. Vitamin C in WBC is the reliable index for the determination of ascorbic acid status.

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