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Chapter-25 Cerebrospinal Fluid Analysis

BOOK TITLE: An Easy Guide for Practical Biochemistry

Author
1. D’sa Divya Shanthi
2. Lakshmi Sowbhagya
ISBN
9789352702633
DOI
10.5005/jp/books/14203_26
Edition
2/e
Publishing Year
2018
Pages
4
Author Affiliations
1. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Subbaiah Institute of Medical Sciences, Shivamogga, Karnataka, India
2. Shimoga Institute of Medical Sciences, Shimoga, Karnataka, India, Sambhram Institute of Medical Sciences and Research, Kolar, Karnataka, India
Chapter keywords
Cerebrospinal fluid, CSF, lumbar puncture, LP, biochemical examination, cytological examination, microbiological examination, neurological disease

Abstract

Examination of cerebrospinal fluid (CSF) is important in the diagnosis of neurological diseases. CSF is usually collected from spinal canal by lumbar puncture (LP). The patient is made to lie on his side with the neck, thighs and knees flexed. The CSF is allowed to flow out spontaneously drop by drop and about 5 mL is collected in clean sterile vials. The sample is used for biochemical, cytological and microbiological examination. CSF must be examined immediately within 1 hour and should not be refrigerated. Biochemical examination of CSF usually consists of measurement of total proteins, glucose and chloride.

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