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Chapter-02 Sample Collection, Liquid-based Cytology and Automated Screening

BOOK TITLE: Handbook of Cervical Cytology: Special Emphasis on Liquid-based Cytology

Author
1. Dey Pranab
ISBN
9789352702657
DOI
10.5005/jp/books/14221_3
Edition
2/e
Publishing Year
2018
Pages
10
Author Affiliations
1. Post Graduate Institute of Medical Education and Research, Chandigarh, India, National Institute of Medical Sciences, Jaipur, Rajasthan, India
Chapter keywords
Cervical smear sampling, liquid-based cytology, LBC, SurePath preparation technique, density gradient sedimentation, conventional preparation, automated screening device

Abstract

The first important step for proper interpretation is optimal collection of cervical cytology specimen. Things needed for cervical cytology smear collection include instrument, fixative, glass slide for conventional preparation (CP), and laboratory form. The patient should be in the dorsolithotomy position as in this position the cervix is better visualized. In case of conventional preparation either broom or spatula is used. The collection of the cervical sampling method is almost same. Presently two companies are available for liquid-based cytology (LBC) i.e. ThinPrep (TP) and SurePath (SP). Unlike smear preparation and fixation in the conventional preparation, these techniques provide the methods of immediate fixation of the cells in liquid fixative. Advantages and disadvantages of LBC preparation, significant differences of the cytology in LBC compared to CP, specific changes in LBC, and automated screening devices are discussed briefly in this chapter.

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