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Chapter-13 Flow Cytometry

BOOK TITLE: Hematopathology Pearls

Author
1. Zhang Da
ISBN
9789352703234
DOI
10.5005/jp/books/14222_14
Edition
2/e
Publishing Year
2018
Pages
42
Author Affiliations
1. The University of Kansas School of Medicine, Kansas City, Kansas, USA, University of Kansas Medical Center, Kansas City, Kansas, USA, University of Kansas Medical Center, Kansas City, Kansas 66160, USA, University of Kansas Medical Center Kansas City, Kansas 66160, USA
Chapter keywords
Flow cytometry, FCM, forward scatter, FSC, hematopoietic cell maturation, flow cytometry phenotyping, chronic lymphocytic leukemia, prolymphocytic leukemia, PLL

Abstract

The basic principle of flow cytometry (FCM) is based on creating a fluid suspension of cells, and then passing through a small capillary tube. A light beam (usually laser) is then directed on the tube. Cells passing through the tube will scatter the light, which is collected by detectors positioned at different angles around the capillary tube. As a cell passes through the tube (and laser beam) multiple parameters can be measured regarding the cell’s size (Forward Scatter, FSC), nuclear complexity/cytoplasmic granularity (Side Scatter, SSC), and surface markers. Applications of flow cytometry, lineage associated markers of flow cytometry phenotyping, hematopoietic cells maturation, and flow cytometry study cases are covered in this chapter.

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