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Chapter-20 Ovarian Tissue Freezing: Introducing New Paradigms in Infertility Preservation

BOOK TITLE: Practical Guide in Reproductive Surgery

Author
1. Dastidar Biswanath Ghosh
ISBN
9789352704842
DOI
10.5005/jp/books/18067_21
Edition
1/e
Publishing Year
2018
Pages
13
Author Affiliations
1. GD Institute for Fertility Research, Kolkata, West Bengal, India, Ghosh Dastidar Institute for Fertility Research, Kolkata, West Bengal, India, Ghosh Dastidar Institute, Kolkata, West Bengal, India
Chapter keywords
Ovarian tissue freezing, infertility preservation, cryopreservation, assisted reproductive technology, ART, fertility preservation, ovarian tissue transplantation, ovarian function

Abstract

Cryopreservation consists of freezing cells or tissues in liquid nitrogen at –196°C for long periods of time without loss of structural and functional integrity. Cryopreservation of sperm and embryos are routinely practiced in reproductive medicine today, although oocyte freezing is a relatively new technological development. Lazzaro Spallanzani is credited to be the first person to freeze and thaw sperm in 1776. The first pregnancy and the first live birth following transfer of embryos derived from frozen oocytes promised much advancement to the field of assisted reproductive technology (ART). This chapter covers the expanding arena of fertility preservation, technical aspects of ovarian tissue cryopreservation, challenges in ovarian tissue cryopreservation, ovarian tissue transplantation, challenges in ovarian transplantation, assessment of ovarian function following transplantation, and complications following transplantation and safety.

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