This chapter discusses cholestatic pathology. This chapter covers the types of bile, flow of bile, formation of bile, role of bile, constituents of bile, important terminologies, etiology, clinical features, laboratory investigation, histological features of cholestasis, and etiological diagnostic consideration in cholestatic disorders. Types of bile are primary bile and secondary bile. Bile formed in liver by hepatocytes and released in canaliculi is known as primary bile. As bile flows through canaliculi into bile ducts, it is modified by absorbtive and secretory processes by cholangiocytes and become bicarbonate rich known as secondary bile. Bile flows from canalicular membrane of hepatocytes into bile canaliculi and then to bile duct in portal tract then into major hepatic ducts.