UG - 13: Genotype and Allele Frequency of Por*28 Gene Polymorphism in South Indian Population

JOURNAL TITLE: Annals of SBV

Author
1. C Adithan
2. Ms. G Pavithra
3. R Mirunalini
ISSN
2395-1982
DOI
10.5005/jp-journals-10085-7114
Volume
7
Issue
1
Publishing Year
2018
Pages
1
Author Affiliations
    1. Mahatma Gandhi Medical College & Research Institute, Pondicherry
    1. Department of Pharmacology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Pondicherry-607402
    1. Central Inter-Disciplinary Research Facility, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth, Pondicherry, India 607 402.
  • Article keywords

    Abstract

    Background: Cytochrome P450 oxidoreductase (POR) supports reactions of microsomal cytochrome P450 which metabolize various drugs and steroid hormones. The principal function of POR is to transfer electrons from reduced NADPH through FMN which then associates with Cytochrome P450 thereby permitting catalysis of the reactions. Polymorphism of POR*28 gene was found to be associated with endocrinological abnormalities, alteration to various drugs, cholesterol metabolism and many other CYP450 metabolizing enzyme activities. The frequency of POR*28 has been studied in various populations like Swedish, Korean and Chinese with varying results. Methodology: This study is a genetic epidemiological study conducted on healthy volunteers. Genomic DNA was isolated from the blood sample using QIAGEN Blood DNA isolation kit. Genotyping of the DNA samples for POR*28 polymorphism was done using TaqMan assay kits in Real Time PCR platform. Results: The results in our study showed a Genotype frequency of Wild type - CC of 41.7% Heterozygous -CT of 47.2% and Mutant - TT of 11.1%. The Allele frequency was C - 65.3% and T - 34.7%. Conclusion: From this study we have identified the genotype and allele frequency of POR*28 gene and the minor allele frequency of POR*28 gene in south Indian population was found to be 34.7%.

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