Influence of Sandblasted and Acid-etched Commercially Pure Grade II Titanium Surface on Human Osteosarcoma Osteoblast Cell Proliferation and Attachment: A Pilot Study

JOURNAL TITLE: International Journal of Prosthodontics and Restorative Dentistry

1. Raj Gaurav Singh
Publishing Year
Author Affiliations
    1. Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, Otago, New Zealand
  • Article keywords
    Human osteoblast osteosarcoma cells, Sandblasted and acid-etched, Titanium


    Aim: To evaluate the effect of surface microtopography on osteogenic cell behavior. Materials and methods: Commercially pure grade II titanium discs of similar design and dimensions (2.5 mm × 6 mm) were selected for the study. Samples were sandblasted using 110 μm grit-size alumina (Al2O3), and 15% hydrofluoric acid (HF), 96% sulphuric acid (H2SO4), and 37% hydrochloric acid (HCl) were used for acid etching. Scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) of samples was performed to observe the surface morphology and elemental analysis. Surface roughness was measured using a surface profilometer. Human osteosarcoma (HOS) cells were seeded at a density of 5 × 103 per test material and control cover slip and incubated for 48 hours. After critical point drying and gold sputtering, a scanning electron microscope was used to observe the cell morphology, proliferation, and cell attachment. Results: Scanning electron microscopy revealed that sandblasting and acid etching resulted in a homogeneous rough surface with a flatter profile. Energy-dispersive X-ray spectroscopy showed a significant increase in the oxygen content (29.38%) after sandblasting and acid etching. Conclusion: Scanning electron microscopy result of the sandblasted and acid-etched surface showed that cell sheets were able to migrate into the pores and adhered inside the valleys suggesting excellent sign of osseointegration.

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