Health science professionals need exhaustive and up-to-date information for patient care and research. They depend much on electronic resources for meeting their information needs. PubMed is a premier open access health science database of US National Library of Medicine (NLM). PubMed indexes more than 5600 health science related journals. PubMed literacy is the information literacy on PubMed database. It helps the health science professionals and researchers in understanding various features of the PubMed database and accessing the required health science information more efficiently and effectively. PubMed literacy means to how to use PubMed for conducting literature search and includes having training in literature search skills and techniques which are essential for searching in PubMed. The present article helps to get familiarity with search features and functionality of PubMed database and to know the importance of PubMed literacy in conducting health science literature search.