Background: Sleep is an indicator of health. Sleep determine many aspects of our life like mood, cognitive functions such as attention and working memory, homeostasis, learning, concentration, etc. Sleep is very essential for the academic progress in college students, whereas most of the sleep-related problems are found in college students worldwide. Aim: This study aimed to evaluate the positive effect of sleep special technique (SST) on sleep quality, stress level and quality of life in young healthy yoga practitioners. Materials and Methods: A total of 120 healthy male yoga practitioners were screened and N = 94 were randomly allocated into two groups, experimental group N = 47, and control group N = 47. Experimental group practiced 1 month of (SST), and the control group had no exposure to SST. Variables Measured: Pittsburgh sleep quality index (PSQI), perceived stress scale (PSS), World Health Organization quality of life–BREF (WHOQOL–BREF), and the vedic personality inventory (VPI) Results: After one month of SST result showed the significant reduction of the global PSQI score (p < 0.001), perceived stress scale (p < 0.001), and considerable improvement in all domains of QOL (p < 0.001), Sattva guna (p < 0.001) in the experimental group compared to the control group. Conclusion: A one-month course of SST has a significantly positive effect on overall sleep quality, quality of life and perceived stress. The transition towards sattva guna was evident during the course of SST intervention.