Aim: The aim of the present study was to compare the remineralizing efficacy of novamin and tricalcium phosphate (TCP). Materials and methods: Nail varnish was coated to a total of 30 sound human premolars except for 5 mm × 5 mm window. Baseline microhardness was measured for all test samples. Artificial carious lesions were created for all teeth by subjecting them to demineralization process. Then microhardness of demineralized lesion was measured. Later artificial caries teeth were equally divided into two groups to treat with remineralization solution for 10 days; group I: novamin and group II: TCP. After 10 days of pH cycling, microhardness was measured. The data were statistically analyzed using Statistical Package for the Social Sciences (SPSS) statistical software from Chicago SPSS Inc., version 21 and using analysis of variance (ANOVA) post hoc multiple comparisons test for intergroup and significant difference at p < 0.05. Results: In the present study, group I indicated a higher value for remineralization compared with group II (p < 0.05). Conclusion: The present study showed that both novamin and TCP were effective in remineralizing the carious lesions. Clinical significance: This study evaluates the remineralizing potential of novamin and TCP on initial carious lesions.