Development of a Novel Polyherbal Formulation for Augmenting Milk Production in Healthy Dairy Cows

JOURNAL TITLE: Journal of Drug Research in Ayurvedic Sciences

1. Jayram Hazra
2. Manish K Gautam
3. Bhavana Srivastava
4. Manish M Wanjari
5. Manajit Bora
6. Sudesh N Gaidhani
7. Rajesh K Tiwari
8. Himanshu Sharma
Publishing Year
Author Affiliations
    1. Central Ayurvedic Research Institute for Drug Development Kolkata, West Bengal, India
    1. Regional Ayurveda Research Institute for Drug Development, Gwalior, Madhya Pradesh, India
    1. Central Council for Research in Ayurvedic Sciences Ministry of AYUSH, Government of India, New Delhi, India
    1. Department of Conservative and Endodontics, Subharti Dental College, Meerut, Uttar Pradesh, India
    1. Central Council for Research in Ayurvedic Sciences, New Delhi, India
    1. Collaborative Research Centre for Veterinary Ayurveda GADVASU, Ludhiana, Punjab, India
    1. Regional Ayurveda Research Institute for Drug Development, Gwalior, Madhya Pradesh, India
  • Article keywords


    Aim: The study was designed to develop and standardize a novel polyherbal formulation (PHF) for augmenting milk production in healthy dairy cattle. Materials and methods: Five raw plant drugs, viz., tubers of Asparagus racemosus Willd. (Shatavari), whole plant of Eclipta alba (L.) Hassk. (Bhringraj), seeds of Trigonella foenum-graecum L. (Methika), fruits of Foeniculum vulgare Mill. (Mishreya.), and Anethum sowa Roxb. ex Fleming (Shatapushpa) were used to prepare hydroalcoholic extracts using the Soxhlet method. Three in-house batches of PHF were prepared and standardized as per Ayurvedic Pharmacopoeia of India (API) methods. Pharmacognostic authentication and chemical identification were done by macroscopic and microscopic studies, phytochemical screening, physicochemical analysis, and high performance thin layer chromatography (HPTLC) fingerprinting. The safety studies of galactagogue preparation were performed through heavy metals, microbial contamination, aflatoxins, and pesticide residue analysis. Results: Organoleptic studies revealed that all the batches appeared as semisolid in nature, blackish-brown in color, with a pleasant odor and slight bitter taste. Phytochemical screening confirmed the presence of similar secondary metabolites in the different batches of both raw drugs and PHF. Physicochemical analysis and HPTLC fingerprints at different illuminations showed that all three batches were uniformly composed and complied the pharmacopeial limits. Results of safety parameters advocated that all the three batches were safe and complied as per the WHO and API guidelines. Conclusion: The present work first claims the standardization of this unique, cost-effective, nonhormonal, Ayurvedic galactagogue in-house preparation, i.e., PHF for augmenting milk yield in dairy herd. It proves that all the three batches have similar characteristics and uniformly composed. It serves as a reference for identification and distinguishing the galactagogue herbs.

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