Abdominal tuberculosis is a most common type of extra-pulmonary tuberculosis. Abdominal tuberculosis can occur primarily or it can be secondary to a tubercular focus elsewhere in the body. Atotal of 153 samples (gastrointestinal biopsies and ascitic fluid) were processed and direct microscopy, culture and Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) were performed. Comparative analysis of the results was done. The overall positivity by culture was 1.3% and by AMTDT was 5.2%.