Introduction: In the world, the violence against women and femicide is an important public health problem and a violation of the human rights, making the women more vulnerable and affecting their physical integrity and mental health. In this way, mechanisms have been established to warrant the gender equality. This study aims to describe the characteristics of external cause of injuries against women and femicide in a first level public hospital of Cali, Colombia between 2012–2014 before and after the implementation of a national public policy of gender equality and deduce about the possible impact on the incidence of these injuries Design and methods: Retrospective observational cohort study. The International Trauma Registry of the Hospital Universitario del Valle was used. It includes all records of women between 14 and 50 years, with trauma injury in the period 2012–2014. A descriptive analysis was performed according to demographic, clinical, and mortality variables. Results: In total of 23,945 records, 3245 (13.6%) are women between 14 and 50 years age with some type of trauma, mean age 29.3 ± 2 years; 57.6% between 19 and 35 years. The injuries associated with an intentionality have been reported in 542 (16.7%) cases. The mortality with intentionality was 3.9% (n = 21). The annual mortality rate for 2012, 2013 and 2014 was 1.2%, 0.8% and 0.3% respectively (p < 0,0001). Conclusion: Femicide was present in 3.9% of cases, in a higher frequency associated to gunshot. However, after the implementation of public policy in 2012, a significant reduction of annual mortality (1.2% vs 0.8% vs 0.3%, p<0,0001) has been observed. It allows to infer that the implementation of the public policy of gender equality facilitated this tendency